Table 1.
Identified sites of citrulline
| Protein | Modified Arginine | Peptide Sequence | Charge | m/z | Pred. m/z | NET Ind. | |
|---|---|---|---|---|---|---|---|
| ACTB | P60709 | 183 | (R)LDLAGrDLTDYLMK(I) | 2 | 812.9162 | 812.9162 | IO |
| 196 | (K)ILTErGYSFTTTAER(E) | 2 | 873.4393 | 873.4389 | RF | ||
| 210 | (R)GYSFTTTAErEIVR(D) | 3 | 544.2738 | 544.2739 | RF | ||
| 2 | 815.9070 | 815.9072 | RF | ||||
| 256 | (R)FrcPEALFQPSFLGMEScGIHETTFNSIMK(C) | 3 | 1179.2135 | 1179.2100 | IO | ||
| 312 | (K)DLYANTVLSGGTTMYPGIADrmQK(E) | 3 | 868.4185 | 868.4209 | IO/RF | ||
| ACTC | P68032 | 30 | (K)AGFAGDDAPrAVFPSIVGRPR(H) | 3 | 719.7115 | 719.7115 | RF |
| 39 | (R)AVFPSIVGrPRHQGVMVGMGQK(D) | 4 | 588.8162 | 588.8161 | RF | ||
| 256 | (K)SYELPDGQVITIGNErFR(C) | 3 | 1048.0225 | 1048.0264 | RF | ||
| 2 | 699.0182 | 699.0200 | RF | ||||
| ARPC3 | O15145 | 34 | (K)GPAPrETKDTDIVDEAIYYFK(A) | 3 | 810.4000 | 810.4006 | IO |
| CDD | P32320 | 5 | (K)rPAcTLKPEcVQQLLVcSQEAK(K) | 3 | 873.7701 | 873.7696 | IO |
| 2 | 1308.6492 | 1308.6508 | IO | ||||
| COR1A | P31146 | 12 | (K)FrHVFGQPAKADQcYEDVR(V) | 4 | 581.7795 | 581.7774 | IO |
| 416 | (R)rAAPEASGTPSSDAVSRLEEEMR(K) | 3 | 816.3864 | 816.3871 | IO/RF | ||
| 453 | (K)RLDrLEETVQAK(–) | 2 | 729.8989 | 729.8992 | IO | ||
| CZIB | Q9NWV4 | 58 | (R)LMDSVALKGGrGSASMVQK(C) | 3 | 646.0028 | 646.0045 | IO |
| ELMO1 | Q92556 | 405 | (R)IVLENSSrEDKHEcPFGR(S) | 4 | 544.2626 | 544.2628 | RF |
| ERH | P84090 | 96 & 97 | (K)IYVLLrrQAQQAGK(–) | 2 | 823.4727 | 823.4728 | IO |
| EZR1 | P15311 | 393 | (R)AQEEAERLEADrMAALR(A) | 3 | 653.9898 | 653.9899 | IO |
| GDIR1 | P52565 | 49 | (K)SIQEIQELDKDDESLrK(Y) | 3 | 683.0105 | 683.0112 | IO |
| HMGB1/2 | P09429 | 24 | (K)MSSYAFFVQTcrEEHKK(K) | 3 | 716.9998 | 716.9996 | IO |
| (K)mSSYAFFVQTcrEEHKK(K) | 3 | 722.3307 | 722.3312 | RF | |||
| HNRPU | Q00839 | 347 | (K)VTEKIPVrHLYTK(D) | 4 | 396.9828 | 396.9832 | IO |
| 558 | (K)LNTLLQrAPQcLGK(F) | 2 | 806.9442 | 806.9456 | IO | ||
| 601 | (K)McLFAGFQrK(A) | 2 | 629.8071 | 629.8072 | IO | ||
| HP1B3 | Q5SSJ5 | 269 | (K)LEDVLPLAFTrLcEPK(E) | 3 | 634.6734 | 634.6729 | IO |
| 293 & 297 | (K)LrVDIrPQLLK(N) | 2 | 676.9167 | 676.9166 | IO | ||
| HSP90A | P07900 | 355 | (R)APFDLFENrK(K) | 2 | 619.3152 | 619.3142 | IO |
| LDHA/B | P00338 | 106/107 | (R)QQEGESrLNLVQR(N) | 2 | 779.4013 | 779.4026 | IO |
| LKHA | P09960 | 587 | (K)DLAAFDKSHDQAVrTYQEHK(A) | 4 | 590.7869 | 590.7871 | IO |
| MOES | P26038 | 408 | (R)QEAEEAKEALLQASrDQK(K) | 3 | 682.3433 | 682.3430 | IO |
| 460 | (K)AQMVQEDLEKTrAELK(T) | 3 | 630.6595 | 630.6594 | RF | ||
| 533 | (K)ALTSELANArDESKK(T) | 3 | 545.2847 | 545.2846 | IO | ||
| MPO H14 | P05164 | 219 | (K)rNGFPVALAR(A) | 2 | 551.3118 | 551.3118 | IO |
| MYH9 | P35579 | 1922 & 1923 | (K)LrrGDLPFVVPR(R) | 3 | 476.2770 | 476.2770 | RF |
| 1191 | (K)TLEEEAKTHEAQIQEMrQK(H) | 3 | 767.3764 | 767.3778 | IO | ||
| 1751 | (R)IAQLEEELEEEQGNTELINDrLK(K) | 3 | 948.1448 | 948.1487 | IO | ||
| 1830 | (K)IAQLEEQLDNETKErQAAcK(Q) | 3 | 792.3874 | 792.3884 | IO | ||
| 1912 | (R)ELEDATETADAMNrEVSSLK(N) | 3 | 737.3418 | 737.3425 | IO | ||
| NCF1 | P14598 | 85 | (K)WFDGQrAAEnRQGTLTEYcSTLMSLPTK(I) | 3 | 1088.1752 | 1088.1763 | IO |
| 85 & 90 | (K)WFDGQrAAENrQGTLTEYcSTLMSLPTK(I) | 3 | 1088.1761 | 1088.1763 | IO | ||
| 340 | (R)RqArPGPQSPGSPLEEER(Q) | 3 | 664.9952 | 664.9960 | IO | ||
| PGM1 | P36871 | 452 | (K)DLEALMFDrSFVGK(Q) | 3 | 543.6045 | 543.6045 | RF |
| 2 | 814.9048 | 814.9031 | IO | ||||
| PLSL | P13796 | 3 | (M)ac-ArGSVSDEEMMELR(E) | 2 | 826.8567 | 825.8664 | IO |
| ROA1 | P09651 | 232 | (R)SrGFGFVTYATVEEVDAAMNARPHK(V) | 4 | 689.3417 | 689.3381 | IO |
| ROA2 | P22626 | 12 | (K)TLETVPLErK(K) | 3 | 396.2277 | 396.2274 | IO |
| S100-A8 | P05109 | 47 | (K)LLETEcPQYIrKK(G) | 3 | 560.2991 | 560.2993 | IO |
| (K)LLETEcPQYIrK(K) | 2 | 775.8961 | 775.8978 | IO | |||
| S10A4 | P26447 | 49 | (R)ELPSFLGKrTDEAAFQK(L) | 3 | 646. 6691 | 646.6707 | IO |
| SH3L3 | Q9H299 | 15 | (R)VYSTSVTGSrEIKSQQSEVTR(I) | 3 | 781.7313 | 781.7311 | IO |
| (R)VYSTSVTGSrEIK(S) | 2 | 714.3721 | 714.3725 | IO | |||
| 51 | (R)IQYQLVDISQDNALRDEMrALAGNPK(A) | 3 | 987.1703 | 987.1696 | IO | ||
| SPTB2 | Q01082 | 1307 | (K)MLTAQDmSYDEArNLHSK(W) | 3 | 709.6548 | 709.6542 | IO |
| TBA4A | P68366 | 21 & 23 | (K)EIIDPVLDrIrK(L) | 2 | 734.9221 | 734.9221 | IO |
| TBB4B/5 | P68371 | 162 | (K)IREEYPDrIMNTFSVVPSPK(V) | 3 | 793.3715 | 793.7386 | IO/RF |
| TLN1 | Q9Y490 | 2538 | (K)FLPSELrDEH(–) | 2 | 622.3012 | 622.3013 | RF |
| 1625 | (R)ALAVNPrDPPSWSVLAGHSR(T) | 3 | 711.0381 | 711.0461 | IO | ||
| VIME | P08670 | 145 | (K)SrLGDLYEEEMRELR(R) | 3 | 500.2326 | 500.2329 | IO |
| 2 | 948.9588 | 948.9596 | IO | ||||
| 304 | (K)FADLSEAANrNNDALR(Q) | 2 | 889.4271 | 889.4268 | RF |
a
r = citrulline (in red)
b
c = alkylated cysteine
c
m = oxidized methionine
d
nq = deamination