Figure 6.

HQH inhibits ERK phosphorylation through inhibition of MEK in Jurkat and Nalm-6 cells. ALL cells were cultured with 50 μmol/L of the MEK inhibitor PD98059 alone, or combination of 4.3 mg/mL HQH for Jurkat cells and 1.5 mg/mL HQH for Nalm-6 cells and PD98059 for 24 hours. We used western blotting to detect the proteins of cleaved-caspase 3, BAX, Bcl-2, t-ERK and pERK in Jurkat (A, C) and Nalm-6 (B, D) cells. β-actin was used as the internal control for western blotting. PD – PD98059; ALL – acute lymphoblastic leukemia; HQH – Huai Qi Huang; pERK – phospho-ERK; t-ERK – total ERK.