Figure 7.

Inhibition of the MEK/ERK pathway enhances GRα expression. ALL cells were cultured with either 50 μmol/L PD98059, 4.3 mg/mL HQH for Jurkat cells and 1.5 mg/mL HQH for Nalm-6 cells or PD98059 combined with HQH. RT-qPCR and western blotting were used to explore the mRNA and protein expression levels of GRα after treatment for 24 hours, respectively, in Jurkat (A, C) and Nalm-6 (B, D) cells. β-actin was used as the loading control for western blotting and GAPDH was used as the internal control for RT-qPCR. * P<0.05, ** P<0.01, *** P<0.001. PD – PD98059; ALL – acute lymphoblastic leukemia; HQH – Huai Qi Huang; RT-qPCR – reverse transcription-quantitative PCR; GR – glucocorticoid receptor.