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. 2020 Feb 15;26:e915825-1–e915825-13. doi: 10.12659/MSM.915825

Figure 4.

Figure 4

There is a target regulatory relationship between miR-122 and DJ-1. (A) Schematic diagram of interaction sites between miR-122 and the 3′-UTR of DJ-1 mRNA. (B) There was a target complementary binding site between miR-122 and the 3′-UTR of DJ-1 mRNA. Double luciferase gene report. White represents miR-NC and deep blue represents miR-122 mimics. * P<0.05 compared with the miR-NC group (white bar is miR-NC and deep blue bar is miR-122mimic) Each experiment was repeated three times. The results of the double luciferase gene report showed that the transfection of miR-122 mimic significantly reduced the relative luciferase activity in the HEK293T cells transfected by pMIR-DJ-1-WT, but there was no significant effect on the relative luciferase activity in the HEK293T cells transfected by pMIR-DJ-1-MUT, indicating that there was a targeting regulation between miR-122 and DJ-1.