Figure 3. Target-adjacent editing by CBE versus MagnEdit.

(A) Quantification of eGFP-positive 293T cells (eGFP Leu202 edited) post-editing and pre-/post-enrichment by FACS for the indicated editing reactions (n = 3 technical replicate experiments, average ± SD). (A, B) Sequence logos summarizing MiSeq data from the same reactions as panel (A). The consensus sequence matches the single-stranded DNA region displaced by gRNA annealing with the target cytosine. Red coloring highlights mutations that occurred in >5% of the MiSeq reads for each reaction (numbers are nucleobase distances 5′ or 3′ of the target “C”).