FIG 3.

ZipA and the three deletions of its unstructured linker domain used in this study. (A) Schematic of ZipA domains, showing the extents of the linker deletions. (B) GFP fusions of ZipA, ZipAΔP/Q, ZipAΔ1/2, and ZipAΔ+/− expressed from pDSW210 were induced with 100 μM IPTG at 30°C, and proteins subjected to SDS-PAGE were probed on Western blots with anti-GFP. Molecular weight markers in kilodaltons are shown at the left. The expected size of each fusion protein is denoted in parentheses, with GFP at 27 kDa. All but ZipAΔP/Q-GFP run abnormally slowly, ∼16 kDa larger than predicted. All lanes were derived from the same blot but were spliced as shown.