FIG 5.

Effects of osmotic or oxidative stress on the ZipA linker mutants. (A) Cells carrying either WT zipA, zipAΔP/Q, or zipAΔ1/2 at the native zipA chromosomal locus were grown to mid-exponential phase in regular LB (0.5% NaCl), LB with no added NaCl (LB 0N), or LB with 0.2 M sucrose and imaged by DIC microscopy. (B) The same strains used for panel A, along with a recA mutant control, were grown in LB supplemented with the indicated concentrations of hydrogen peroxide for 2.5 h at 30°C, and then 10-fold dilutions were spotted onto LB plates and incubated overnight at 30°C.