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FtsA is more toxic in zipA1 cells than in WT cells. WM1074 or WM5337 (MG1655 zipA1) cells carrying either pDSW210F empty vector (Ev) or pDSW210F-FtsA under IPTG control were serially diluted 10-fold, spotted on plates containing either glucose (to suppress gene expression) or the indicated amounts of IPTG (in micromolar), and incubated overnight at 30°C.
