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Overproduced FtsA is less toxic in zipAΔP/Q cells than in WT cells or in cells with zipAΔ1/2. WM1074 (WT) or WM1074 derivatives with chromosomal zipA replaced with either zipAΔP/Q or zipAΔ1/2 carrying either pDSW210F empty vector or pDSW210F-FtsA under IPTG control were serially diluted 10-fold, spotted on plates containing the indicated amounts of IPTG (in micromolar), and incubated overnight at 30°C.
