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Neither ZipA nor the linker deletions of ZipA confer significant suppression of several key fts mutants. MG1655 derivatives containing various pDSW210 plasmid derivatives of ZipA or ZipA linker mutants in strains with the ftsK44 (A), ftsZ84 (B), or ftsI23 (C) thermosensitive allele were grown at 30°C, serially diluted 10-fold, and spotted onto plates at the permissive (30°C) or nonpermissive (42°C) temperature with various levels of IPTG to induce the ZipA constructs.
