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Increased sensitivity of the ZipA linker mutants to the effects of excess FtsEX. WT (WM1074) or various mutant derivatives of WM1074 carrying the IPTG-inducible pDSW207-FtsEX plasmid were grown to mid-exponential phase, diluted 10-fold, spotted onto LB plates supplemented with various concentrations of IPTG, and incubated overnight at 30°C.
