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. 2020 Feb 25;202(6):e00720-19. doi: 10.1128/JB.00720-19

TABLE 2.

Plasmids used in this study

Plasmid Description Source or reference
pDSW210 Ptrc-gfp pBR322 derivative 58
pKD46 Expresses λRed functions 51
pWM2784 pDSW210F (with N-terminal FLAG tag) 59
pWM2785 pDSW210F-ftsA 59
pWM5741 pDSW210F-ftsA recloned WT linker This study
pWM5742 pDSW210F-ftsAΔ10 This study
pWM5743 pDSW210F-ftsAΔ11 This study
pWM5744 pDSW210F-ftsAΔ9–10 This study
pWM5745 pDSW210F-ftsAΔ11–12 This study
pWM5746 pDSW210F-ftsAΔ8–10 This study
pWM5747 pDSW210F-ftsAΔ11–13 This study
pWM5749 pDSW210F-ftsAΔ11–14 This study
pWM5870 pDSW210F-ftsAΔ6–10 This study
pWM5871 pDSW210F-ftsAΔ8–13 This study
pWM5872 pDSW210F-ftsAΔ8–15 This study
pWM6031 pDSW210F-ftsAΔ1–20 This study
pWM6032 pDSW210F-ftsA scrambled linker sequence This study
pWM6059 pDSW210-zipAΔP/Q This study
pWM6060 pDSW210-zipAΔ+/− This study
pWM6061 pDSW210-zipAΔ1/2 This study
pWM6062 pDSW210F-ftsAΔ5–15 This study
pWM6063 pDSW210F-ftsAΔ5–19 This study
pDSW207 pDSW210 derivative with stronger trc promoter 58
pWM2799 pDSW207-ftsEX Lab collection
pKG116 pACYC184 derivative with nahG promoter 60
pWM5779 pKG116-ftsA Lab collection