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. 2020 Feb 26;15(2):e0229744. doi: 10.1371/journal.pone.0229744

Fig 4. Hdc and Klf4 expression during BMMC differentiation in the absence or presence of DFMO.

Fig 4

(A) During BMMC differentiation, cells on day 7, 14, 21, and 28 were collected and used to prepare cell lysates. HDC assays were performed after dialysis of the cell lysate. Mean ± s.d. is shown, n = 3 replicates. *p < 0.05 compared with control. (B) Total RNA extracted from cells on day 7, 14, and 21 was isolated and used for qRT-PCR. Relative expression of Hdc mRNA was normalized to Gapdh and control expression is shown as 1. Mean ± s.d. is shown, n = 3 replicates. *p < 0.05 compared with control. (C) During BMMC differentiation, cells on day 7, 14, 21, and 28 were collected and analyzed for KLF4 and β-actin by western blotting. Lanes (C, D, and DP) represent control, DFMO, and DFMO + PUT, respectively. (D) Total RNA from cells on day 7, 14, and 21 was isolated and used for qRT-PCR. Relative expression of Klf4 mRNA was normalized to Gapdh; control expression is shown as 1. Mean ± s.d. is shown, n = 3 replicates. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with control.