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. 2020 Feb 26;15(2):e0229534. doi: 10.1371/journal.pone.0229534

Fig 4. The effect of TMZ on total DNA methylation of U138 cell line.

Fig 4

The analysis was performed after 3 (white bars), 12 (light grey bars), 24 (dark grey bars) and 48 (black bars) hrs of incubation in a given TMZ concentration (0.5–100 μM). Control cells (C) were treated with DMSO only. The R values are means from three experiments ±SD. Asterisks indicate a significant difference (*p < 0,05, **p < 0,01, ***p < 0,001) from the control (DMSO) value. A very little DNA hypermethylating effect is observed (for incubation times 3 and 12 hrs, over concentrations 20 and 30 μM respectively). Very clear initial hypomethylation is seen in TMZ concentrations up to 100 μM for incubation times of 24 and 48 hrs.