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. 2020 Feb 26;15(2):e0229534. doi: 10.1371/journal.pone.0229534

Fig 10. The impact of TMZ exposure period on DNA methylation in U118 cell line.

Fig 10

U118 cell culture was treated with the given single dose of TMZ (0.5, 1, 3, 5, 10 μM) and left for 1–7 days. The global DNA methylation was estimated after 1st, 2nd, 3rd, 4th, 5th, and 7th day of incubation. A general hypermethylation is observed, with no initial hypomethylating effect in lower concentration range. Hypomethylation after 7 days starts with concentrations over 1 μM.