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. 2020 Feb 26;15(2):e0229570. doi: 10.1371/journal.pone.0229570

Fig 4. IL-18 produced by THP-1 cells after infection with UV-irradiated HSV-1.

Fig 4

A. THP-1 cells were stimulated overnight with PMA (5 ng/mL) and then incubated with HSV-1, UV irradiated HSV-1 (HSV-1/UV), or media for 24 hours. Cell culture supernatants were collected and IL-18 was measured via ELISA. B. THP-1 cells were stimulated with PMA (5 ng/mL) and then with IFN0γ (25 ng/mL) the following day for 24 hours prior to incubation with HSV-1, UV irradiated HSV-1 (HSV-1/UV), or media alone for 24 hours. Cell culture supernatants were collected and IL-18 was measured via ELISA. ΔHUMCYC cells are labeled as “WT.” Differences between indicated conditions within a cell type were determined by a one-way ANOVA with Tukey HSD post-hoc analysis. NS, *,**,*** indicate p-values >0.05, <0.05, <0.01, <0.001 respectively.