Fig 5. IL-1β produced by THP-1 cells after infection with UV-irradiated HSV-1.

A and B. THP-1 cells were stimulated with PMA (5 ng/mL) and then with IFNγ (25 ng/mL) the following day for 24 hours prior to incubation with HSV-1, UV irradiated HSV-1 (HSV-1/UV), or media alone for 24 hours. Cell culture supernatants were collected and IL-1β was measured. ΔHUMCYC cells are labeled as “WT.” Differences between indicated conditions within a cell type were determined by a one-way ANOVA with Tukey HSD post-hoc analysis. NS, *,**,*** indicate p-values >0.05, <0.05, <0.01, <0.001 respectively.