Figure 7.

Cytokine assessment in the supernatant of spleen cell cultures. Extracted spleen cells were stimulated with soluble Leishmania antigen (25 μg/mL) for 48 hr and 72 hr for IL-10 and IFN-γ, respectively. The levels of cytokines were determined using ELISA. Tukey’s test followed by one way ANOVA was used to perform statistical analysis (* P≤0.05); A. increased level of the IFN-γ level in vaccinated groups before challenge; B. Higher level of IL-10 in mice vaccinated with pleish-dom alone before challenge; C. 4-fold increasing of IFN-γ level in mice vaccinated with pleish-dom/pIL-12 than before challenge; D. elevated level of IL-10 in control groups at 8 weeks post-challenge compared to before challenge