Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2021 Mar 1.

Published in final edited form as: J Leukoc Biol. 2019 Nov 1;107(3):367–377. doi: 10.1002/JLB.1HI1019-116RR

Figure 4. — A. Serum eotaxin-1 in WT (wild-type) and eotaxin-1 gene-deleted mice; n = 3 – 5 mice, ***p < 0.005, Mann-Whitney u-test. B. Bone marrow-derived eosinophils cultures initiated under standard conditions as indicated; at day 4, these factors are replaced with 0.5 ng/mL IL-5 alone, 0.5 ng/mL IL-5 with 0.5 ng/mL eotaxin-1, or 0.5 ng/mL eotaxin-1 alone; total cells in culture on each day are as shown; n = 3 – 5 per point, ***p < 0.005. C. SiglecF⁺ cells (% of total) at day 12 of culture under growth conditions indicated; n = 5 – 8 per point; no significant differences detected between groups. D. Gr1⁻ cells (% of SiglecF⁺) in bmEos cultures under growth conditions indicated; E. CD125⁺ (IL5-Rα⁺) cells (% of SiglecF⁺) in bmEos cultures as above. F. CD34⁺ cells (% of SiglecF⁺) in bmEos cultures grown as above. G. CCR3⁺ cells (% of SiglecF⁺) in bmEos cultures as above; for D. – G., n = 5 – 8 per point, *p < 0.05, **p < 0.01; ***p < 0.005, ns, not significant, 1-way ANOVA.

Figure 4. — A. Serum eotaxin-1 in WT (wild-type) and eotaxin-1 gene-deleted mice; n = 3 – 5 mice, ***p < 0.005, Mann-Whitney u-test. B. Bone marrow-derived eosinophils cultures initiated under standard conditions as indicated; at day 4, these factors are replaced with 0.5 ng/mL IL-5 alone, 0.5 ng/mL IL-5 with 0.5 ng/mL eotaxin-1, or 0.5 ng/mL eotaxin-1 alone; total cells in culture on each day are as shown; n = 3 – 5 per point, ***p < 0.005. C. SiglecF⁺ cells (% of total) at day 12 of culture under growth conditions indicated; n = 5 – 8 per point; no significant differences detected between groups. D. Gr1⁻ cells (% of SiglecF⁺) in bmEos cultures under growth conditions indicated; E. CD125⁺ (IL5-Rα⁺) cells (% of SiglecF⁺) in bmEos cultures as above. F. CD34⁺ cells (% of SiglecF⁺) in bmEos cultures grown as above. G. CCR3⁺ cells (% of SiglecF⁺) in bmEos cultures as above; for D. – G., n = 5 – 8 per point, *p < 0.05, **p < 0.01; ***p < 0.005, ns, not significant, 1-way ANOVA.