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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Leukoc Biol. 2019 Nov 1;107(3):367–377. doi: 10.1002/JLB.1HI1019-116RR

Figure 5. Interleukin-5 and eotaxin-1 contribute to the functional phenotype of bone marrow derived eosinophils.

Figure 5.

A. Chemotaxis in response to increasing concentrations of eotaxin-1 (10 to 1000 ng/mL). Values shown are ratios of cells migrated in response to eotaxin-1 to cells migrated in response to medium alone. Growth conditions for bmEos cultures (10 ng/mL IL-5, 0.5 ng/mL IL-5, 0.5 ng/mL IL5 with 0.5 ng/mL eotaxin-1 (10 days) are as indicated; **p < 0.01, ***p < 0.005 vs. 10 ng/mL IL5 cultures, 2-way ANOVA. B. BmEos grown for 10 days in 0.5 ng/mL IL-5 with 0.5 ng/mL eotaxin-1 were separated into SiglecF+Gr1− and SiglecF+Gr1+ fractions and chemotaxis in response to increasing concentrations of eotaxin-1 (10 – 1000 ng/mL) was evaluated. Ratios as in A. are shown; n = 5 per group, *p < 0.05, 1-way ANOVA. C. Percent survival of bmEos grown in culture as above, n = 3 per point, *p < 0.05, **p < 0.01, ***p < 0.005, 2-way ANOVA.