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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Curr Biol. 2020 Jan 30;30(4):708–714.e4. doi: 10.1016/j.cub.2019.12.021

Figure 3. Unwrapped PGCs are cannibalized by endodermal cells.

Figure 3.

(A) Time-lapse sequence showing PGC death in a lam-1(RNAi) embryo (PGC before death, chevron; degrading PGC, arrowheads). Times are hours:min relative to gonad primordium formation. (B) Relative positions of SGPs, PGCs and endodermal cells in control and SGP-ablated (∅ SGPs) late-stage embryos. Arrowheads, engulfed (middle panel) or degrading (right panel) PGC cell body. (C) PGC position at the time of gonad assembly in control and SGP-ablated embryos. Dashed line, midline. (D) Analysis of variance in PGC position in control and SGP-ablated embryos, compared using an F-test for equality of variance (***p≤0.001; control, n=26; SGP-ablated, n=35). Mean(bar) and S.D. are indicated. (E) end-1 end-3 SGP-ablated embryo showing surviving PGC cell bodies (arrowheads) and persistent lobes. (F-G) PGC cell body (arrowhead, identified by DIC) inside of endodermal cells in ced-10 and lst-4 mutant SGP-ablated embryos. Arrow, unengulfed lobes. Scale bars, 5 μm. See Figure S4.