Cytc acts as an electron carrier between complex III (bc1 complex) and complex IV (cytochrome c oxidase) in the ETC resulting in efficient ATP production at complex V (ATP synthase). Under basal conditions, Cytc is phosphorylated in a tissue-specific manner. Phosphorylated Cytc is less likely to get released from the mitochondria and activate apoptosis in the cytosol. Under conditions of cellular stress, such as ischemia-reperfusion injury, dephosphorylated Cytc is eventually released from the mitochondria via channels including Bax/Bak pores. In the cytosol dephosphorylated Cytc interacts with Apaf-1 forming the apoptosome, which then activates capase-9 and the downstream caspase cascade, executing cell death. OMM; outer mitochondrial membrane, IMS; intermembrane space, IMM; inner mitochondrial membrane.