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Primer sequences used in real-time PCR
| Gene | Primer sequences | Product size |
|---|---|---|
| GADPH | 5’-GCAAGAGAGAGGCCCTCAG-3’ 5’-TGTGAGGGAGATGCTCAGTG-3’ |
74 |
| IL-1β | 5’-CACCTCTCAAGCAGAGCACAG-3’ 5’-GGGTT CCATGGT GAAGTCAAC-3’ |
79 |
| TNF-α | 5’-CCAGGAGAAAGTCAGCCTCCT-3’ 5’-TCATACCAGGGCTTGAGCTCA-3’ |
87 |
| IL-4 | 5’-TCCTTACGGCAACAAGGAAC-3’ 5’-GT GAGTTCAGACCGCT GACA-3’ |
181 |
| IL-10 | 5’-CACTGCTATGTTGCCTGCTC-3’ 5’-TT CATGGCCTT GT AGACACC-3’ |
463 |
