Fig. 2.

CB1 knockout reduced hepatic PLIN2 in HBs transgenic mice. a Quantitative real-time PCR analysis of PLIN2 revealed reduced PLIN2 mRNA in CB1^−/− and HBS/CB1^−/−-mice. Data were normalized to β-actin as reference gene and shown as relative expression (ΔΔCt). b Western blot analysis of total protein lysates from the liver of 52-week-old mice demonstrates reduced PLIN2 protein levels in HBs/CB1^−/− mice. c Immunofluorescence analysis of paraffin-embedded liver sections from 52-week-old mice was performed using specific anti-PLIN2 antibody (red). Nuclei were stained with DAPI (blue). Original magnification ×1000, bar 40 µm