Fig. 3.

Pharmacologic antagonization of CB1 reduced PLIN2 expression in cell culture a AML12 cells were treated with rimonabant (rimo) or methanadamide (meth-AEA) at 1 µM final concentration for 48 h in the absence or presence of oleic acid (200 µM). Western blot analysis demonstrated enhanced PLIN2 expression with meth-AEA and reduced PLIN2 expression with rimo-treatment. Equal protein loading was confirmed using anti-GAPDH antibody. A representative Western Blot is shown. b Immunofluorescence analysis of PLIN2 (red) in AML12 cells treated with rimonabant or meth-AEA. Nuclei were stained with DAPI (blue). Representative stainings are shown. Original magnification ×400, bar 100 µm. c Dose dependent decrease in PLIN2 expression in AML12 cells treated with rimonabant at different doses (0.1, 0.5, 1, and 2 µM), respectively. in the presence of oleic acid (200 µM). Cell lysates were analyzed by western blot using specific anti-PLIN2 antibodies. Equal protein loading was confirmed using an anti-GAPDH antibody