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. 2019 Nov 26;39(9):1983–1996. doi: 10.1038/s41388-019-1120-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overexpression of miR-200a in human BCs and BBN-treated mouse invasive BCs, and its specific promotion of cell invasion in human invasive BC cell lines. a, b miR-200a expression levels analyzed by real-time PCR in human and mouse BC tissues. c miR-200a expression was analyzed by real-time PCR in UROtsa, T24T, and UMUC3 cell lines. d–i miR-200a expression analyzed by real-time PCR in T24T(Vector) vs. T24T (miR-200a inhibitor) (d) and UMUC3(Vector) vs. UMUC3(miR-200a inhibitor) (g) cells. Invasive abilities of T24T (Vector) vs. T24T (miR-200a inhibitor) (e, f) and UMUC3(Vector) vs. UMUC3(miR-200a inhibitor) (h, i) were analyzed in Invasion Chambers. j–o miR-200a expression was analyzed by real-time PCR in T24T (Vector) vs. T24T (miR-200a) (j) and UMUC3(Vector) vs. UMUC3(miR-200a inhibitor) (m) cells. The invasive abilities of T24T(Vector) vs. T24T(miR-200a) cells and UMUC3(Vector) vs. UMUC3(miR-200a) cells were determined in invasion chambers; and the results were presented as relative invasion in the two paired transfectants, respectively (k, l, n, o). Bars represent means ± SD of three independent experiments. *p < 0.01