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. 2020 Feb 20;14:5. doi: 10.3389/fnana.2020.00005

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Copyright © 2020 Sivalingam, Ogawa and Parhar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of morphine on oprm1 mRNA expression in the brain of zebrafish. Fish were individually immersed in morphine 2 mg/L (treated) or water (control) for 20 min and then the brain samples were collected. (A–D′) Photomicrographs showing expression of oprm1 genes. (A; control and A′; morphine treated) Medial zone of the dorsal telencephalic area and dorsal nucleus of the ventral telencephalic area, (B,B′) ventral nucleus of the ventral telencephalic area, (C,C′) posterior part of preoptic area, and (D,D′) ventral periventricular hypothalamus. N = 6 each group. Scale bars: (A–D) and (A′-D′) (100 μm).