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. 2020 Feb 20;14:5. doi: 10.3389/fnana.2020.00005

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Copyright © 2020 Sivalingam, Ogawa and Parhar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of npas4a gene in the brain of zebrafish. Photomicrographs showing the mRNA expression of neural activity marker, npas4. (A) Lines on the schematic sagittal drawing view of the zebrafish brain indicate levels of coronal sections (B; control and B′; morphine treated) medial zone of dorsal telencephalic area, (C,C′) ventral nucleus of ventral telencephalic area, (D,D′) preoptic area, (E,E′) habenula, and (F,F′) ventral periventricular hypothalamus. N = 6 each group. Scale bars: (B–F) and (B′-F′) (100 μm).