FIGURE 5.

SAR7334 reduced [Ca²⁺]_i and [Na⁺]_i and blocked the elevation of [Ca²⁺]_i and [Na⁺]_i induced by OAG. [Ca²⁺]_i was measured in VSMc isolated from Wt and mdx mice before and after incubation in SAR7374 (0.1 μM or 1 μM), as well as after the exposure with SAR7374 and OAG (100 μM). (A) shows that preincubation in SAR7374 (0.1 μM) reduced significantly [Ca²⁺]_i and [Na⁺]_i in both genotypes. (B) illustrates that SAR7374 (1 μM) further reduced [Ca²⁺]_i and [Na⁺]_i and prevented the increase in intracellular [Ca²⁺] and [Na⁺] induced by OAG. (C) shows that SAR7374 (1 μM) reduced [Na⁺]_i in control and mdx VSMc and prevents the elevation of [Na⁺]_i induced by OAG. Over the horizontal axis are indicated the experimental conditions used to measure [Ca²⁺]_i and [Na⁺]_i. For [Ca²⁺]_i: n_mice = 3/experimental condition, n_cell = 17–20/genotype. For [Na⁺]_i: n_mice = 3/experimental condition, n_cell = 13–19/genotype. Values are expressed as means ± S.D. for each condition. One-way ANOVA with Tukey’s post-test, **p < 0.01, ***p ≤ 0.001.