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. 2020 Feb 26;11:1050. doi: 10.1038/s41467-020-14885-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Loss of function in the fusion gene fzo-1 suppresses wound closure defects of gtl-2(n2618) and mcu-1(ju1154) mutants. Quantitation of actin ring diameter in WT and mutants 1 h after needle wounding (WT, n = 50; gtl-2, n = 27; mcu-1, n = 33; fzo-1, n = 64; gtl-2;fzo-1, n = 20; fzo-1;mcu-1, n = 33 animals). gtl-2 and mcu-1 mutants display delayed actin ring closure, which is suppressed in double mutants with fzo-1(tm1133). The WT actin ring diameter was normalized to 1 and mutants normalized to WT. Bars indicate mean ± SEM. *P = 0.0328, ***P = 0.0001, ****P < 0.0001, One-way ANOVA Dunnett’s test (single mutant versus WT). two-tailed unpaired t-test (versus mcu-1 or gtl-2). b Quantitation of actin ring diameter in the mitochondrial mutants (WT, n = 34; drp-1, n = 54; miro-1, n = 47; fzo-1, n = 44; miro-1;fzo-1, n = 82; fzo-1;drp-1 RNAi, n = 39 animals). drp-1 partially suppresses fzo-1 while miro-1 significantly suppresses the fzo-1 in enhanced wound closure. Bars indicate mean ± SEM. ns, P = 0.7753 (drp-1) or 0.9893 (miro-1), ***P = 0.0002, ****P < 0.0001, two-tailed unpaired t-test (versus fzo-1). One-way ANOVA Dunnett’s test (single mutant versus WT). Source data are provided as a Source Data file. c Representative confocal images of mitochondrial morphology in fzo-1 and miro-1;fzo-1 mutants. Pcol-19-Tomm-20::GFP(zjuSi48) transgenic animals label the mitochondria. fzo-1 mutants display fragmented and round shaped mitochondria. miro-1;fzo-1 double mutants have tubular mitochondria, similar to miro-1 single mutants. N = 3 independent experiments. d Representative confocal images of mitochondrial morphology in fzo-1 and drp;fzo-1 mutants. Mitochondria were labeled with mito::dendra2(juSi271), the mitochondrial morphology of drp-1;fzo-1 mimics drp-1, without fragmented mitochondria. Note, drp-1 suppress fzo-1 in mitochondrial morphology. Scale (c, d), 10 μm and 5 μm (zoom-in image).