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. 2020 Feb 26;11:1050. doi: 10.1038/s41467-020-14885-x

Fig. 6. The upregulation of oxidative signals in fzo-1 mutants is required for faster wound closure.

Fig. 6

a Venn diagram of differentially expressed genes (DEGs) in WT needle wounded and fzo-1(tm1133) unwounded animals, compared to WT unwounded controls, as measured by RNA-seq (Benjamini-Hochberg adjusted P value <0.001). See also Supplementary Data 1. b Gene expression heatmap of 216 DEGs shared by WT wounded and fzo-1 unwounded animals as described in a. c DAVID biological process GO terms of upregulated and downregulated genes in the 125/216 overlapping DEGs (Benjamini-Hochberg adjusted P value <0.001). See also Supplementary Data 2. d Heatmap of 19 overlapping oxidation-reduction processes genes. e–l Quantification of oxidation-reduction process-related genes in the WT, wounded, and fzo-1 animals as determined by qPCR. In both WT wounded and fzo-1 mutant, cyp-13A8, cyp-13A12, cyp-14A4, cyp-33D3, gst-20, dhs-23, and dhs-28 were upregulated while acdh-2, dhs-25 are down-regulated. N = 3 independent experiments; Bars indicate mean ± SEM. m Representative confocal images of actin ring formation 1 h.p.w. RNAi knockdown of cyp-13A8 and cyp-13A12 suppresses the accelerated actin ring formation in fzo-1(tm1133). Scale bar, 10 μm. N = 3 independent experiments. n Quantitation of actin ring formation percentage in the WT and fzo-1(tm1133) double mutants with RNAi knockdown cyp-13A8, and cyp-13A12 genes. N = 50, ****P < 0.0001, versus WT or fzo-1(tm1133), Two-sided Fisher’s exact test. o Quantitation of actin ring diameter in the WT and fzo-1(tm1133) double mutants with RNAi knock down cyp-13A8 (WT-L4440, n = 17; WT-cyp-13A8, n = 17; fzo-1-L4440, n = 19; fzo-1;cyp-13A8, n = 13 animals). Bars indicate mean ± SEM, ns, P = 0.803, ****P < 0.0001, versus WT or fzo-1(tm1133). two-tailed unpaired t-test. p Overexpression of CYP-13A8 in epidermis accelerates wound closure. Left, representative actin ring images, Scale bar, 10 μm. Right, quantitation of actin ring diameter 1 h.p.w (WT, n = 28; Pcyp-13A8-cyp-13A8, n = 21; Pcol-19-cyp-13A8, n = 19 animals). Bars indicate mean ± SEM, ****P < 0.0001, versus non transgenic animals. One-way ANOVA Dunnett’s test. Source data are provided as a Source Data file.