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. 2020 Feb 20;11:197. doi: 10.3389/fimmu.2020.00197

Figure 2.

Figure 2

A 129 locus on chromosome 6 in C57BL/6 mice impairs the anti-LCMV CD4+ T cell response. (A) C57BL/6 and two different lines of CD1d-KO mice (CD1d1tm1Luc and CD1d1tm1.1Aben) (n = 3–5 mice/group) were infected i.c. with 1 × 103 p.f.u. LCMV Armstrong 3. The mice were sacrificed on day 8 post-infection, cervical lymph nodes (cLN) were harvested, single cells were stained for CD4, CD44, CD16/32, and IAbgp-61 tetramer, and analyzed by flow cytometry. Data show the total number of cells that are CD4+ CD16/32– CD44hi IAbgp-61tet+ (±SE). **p ≤ 0.01, (Student's t-test). (B) Dot plots show the expression of NKp46 and NK1.1 on cLN cells that are TCRβ-. (C) SNP analysis was done on CD1d1tm1.1Aben mice that were NK1.1hi (n = 5) and CD1d1tm1Luc mice that were either NK1.1int (n = 5) or NK1.1low (n = 6). (D,E) C57BL/6, CD1d-KO.NKC129, BL/6.NKC129, and 129SvJ mice (n = 3–5 mice/group) were sacrificed on day 8 post-LCMV infection. Data show the total number of cells that are CD4+ CD16/32– CD44hi IAbgp-61tet+ (±SE). **p ≤ 0.05, (Mann–Whitney test) and are representative of three independent experiments with similar results.