Figure 4.

Dendritic cell antigen-presentation is impaired in C57BL/6 mice with the NKC¹²⁹ locus and is restored in the absence of perforin. (A,B) Groups of C57BL/6 and BL/6.NKC¹²⁹ mice (n = 3–4 mice/group) were infected i.c. with 1 × 10³ p.f.u. LCMV Armstrong 3 and were injected with either PBS or anti-asialo-GM1 (days −2, 2) and sacrificed on day 4 after infection. Single cell suspensions from cLN were generated, and cells were stained with antibodies to characterize dendritic cells (DCs). Graphs show (A) the frequency of PDCA-1+ cells (gated from CD11b+CD11c+) on day 4 after infection, and (B) after NK cell depletion. (C,D) C57BL/6 and CD1d-KO.NKC¹²⁹ mice (n = 3–5 mice/group) were infected i.c. with 1 × 10³ p.f.u. LCMV Armstrong 3. CD11c+ cells were isolated from the cLN on day 6 post-LCMV infection and co-cultured with CFSE labeled naïve SMARTA CD4+ T cells for 72 h. Data show (C) histograms and (D) graph of the frequency of SMARTA CD4+ T cells that are CFSE^lo that were cultured with DCs from either C57BL/6 or CD1d-KO.NKC¹²⁹ mice (±SE). Data are representative of 2 independent experiments with similar results. (E) C57BL/6, BL/6.NKC¹²⁹, CD1d-KO.NKC¹²⁹, and CD1dKOxPfp^−/−.NKC¹²⁹ mice (n = 3–5 mice/group) were infected i.c. with 1 × 10³ p.f.u. LCMV Armstrong 3. CD11c+ cells were isolated from the cLN on day 6 post-LCMV infection and co-cultured with CFSE labeled naïve SMARTA CD4+ T cells for 72 h. Data show the frequency of SMARTA CD4+ T cells that are CFSE^lo (±SE) across multiple T cell:DC ratios. **p ≤ 0.01 (Student's t-test).