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. 2020 Feb 26;11(2):151. doi: 10.1038/s41419-020-2350-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Cellular extracts from MCF-7 cells stably expressing FLAG-SET7/9 or the vector alone were immunopurified with an anti-FLAG affinity column and eluted using FLAG peptides. The eluates were resolved by SDS–PAGE and analyzed by mass spectrometry. b The column-bound proteins were analyzed by western blot using antibodies against TRIM21. c Co-immunoprecipitation analysis was performed in MCF-7 cells, T-47D cells, MDA-MB-231 cells, and MCF-10A cells with antibodies against SET7/9. Immunocomplexes were then immunoblotted using antibodies against TRIM21, and the reciprocal procedure was also performed. d The GST pull-down assay was performed with GST-SET7/9 or its deletion mutants and in vitro-transcribed and translated full-length TRIM21.