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. 2020 Feb 26;11:1053. doi: 10.1038/s41467-020-14905-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Hypocotyl lengths of seedlings grown under the continuous white light at 20 °C for 7 days. Error bars indicate s.d. (n = 15 plants). **P<0.01 (two-tailed Student’s t-test); NS, not significant (two-tailed Student’s t-test P ≥ 0.05). b qRT-PCR analysis of PIF4. Seedlings were grown in the same conditions as a. The expression levels of PIF4 were normalized to those of PP2A. Error bars indicate s.d. (n = 3). *P < 0.05 and **P < 0.01 (two-tailed Student’s t-test). c Hypocotyl lengths of seedlings grown under continuous white light at 20 °C for 7 days. Error bars indicate s.d. (n = 15 plants). **P < 0.01 (two-tailed Student’s t-test). d PIF4 expression levels in the seedlings grown in the same growth conditions as c. The expression levels of PIF4 were normalized to those of PP2A. Error bars indicate s.d. (n = 3). *P < 0.05 and **P < 0.01 (two-tailed Student’s t-test). e The Venn diagram shows the overlap between genes that are up-regulated by global PIF quartet (G.PIFQ), ML1 promoter-driven PIF4 (ML1:PIF4 UP), and SUC2 promoter-driven PIF4 (SUC2:PIF4 UP). f The Venn diagram shows the overlap between genes that are down-regulated by the global PIF quartet, ML1:PIF4, and SUC2:PIF4. g GO analyses of ML1:PIF4-regulated and SUC2:PIF4-regulated genes. Total: Arabidopsis total genes. h Percentage of auxin (picloram)-activated or repressed genes in global PIF quartet-regulated or ML1:PIF4-regulated or SUC2:PIF4-regulated genes. i Percentage of PIF4 direct target genes in ML1:PIF4-regulated or SUC2:PIF4-regulated genes. Source data are provided as a Source Data file.