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. 2020 Feb 26;11:1053. doi: 10.1038/s41467-020-14905-w

Fig. 3. Epidermal PIF4 activity is required for thermoresponsive hypocotyl growth.

Fig. 3

a Hypocotyl lengths of seedlings grown under continuous white light at 20 °C for 7 days or 20 °C for 4 days followed by 28 °C for 3 days. Error bars indicate s.d. (n = 15 plants). The P-value for the interaction term (genotype x temperature) calculated by two-way ANOVA is shown at the top. Black asterisks above the bars (in a-h) indicate significant differences (**P < 0.01 and *P < 0.05, two-tailed Student’s t-test). NS, not significant (P ≥ 0.05). Numbers above the bars (in a, d, f) indicate the ratio of hypocotyl lengths of seedlings grown at two different temperatures (28 °C/20 °C). b, c qRT-PCR analyses of YUC8 and ATHB2 expression. Seedlings were grown in 12 h light/12 h dark cycles (12 L:12D) at 20 °C for 4 days and transferred under the continuous white light on 5th day. The growth temperature was increased to 28 °C or kept at 20 °C for 4 h at Zeitgeber Time (ZT) 20-24 before harvesting for total RNA extraction. Gene expression levels were normalized to APX3 and presented as values relative to that of the wild type (WT) kept at 20 °C. Error bars indicate s.d. (n = 3). The P-value for the interaction (genotype x temperature) is shown at the top. d Hypocotyl lengths of WT, pifq, and ML1p::PIF4mi seedlings grown under continuous white light at 20 °C for 7 days or 20 °C for 4 days followed by 28 °C for 3 days. Error bars indicate s.d. (n = 15 plants). The P-value for the interaction (genotype x temperature) is shown at the top. e, f Hypocotyl lengths of WT, ML1p::PIF4ΔN-SRDX, and SUC2p::PIF4ΔN-SRDX seedlings grown under continuous white light at 20 °C for 7 days or 20 °C for 4 days followed by 28 °C for 3 days. Representative seedlings are shown in e and error bars in f indicate s.d. (n = 15 plants). The P-value for the interaction (genotype x temperature) is shown at the top. g, h qRT-PCR analyses of YUC8 and IAA19. WT and transgenic seedlings were grown in 12 h light/12 h dark cycles (12 L:12D) at 20 °C for 4 days and transferred under the continuous white light on 5th day. The growth temperature was increased to 28 °C or kept at 20 °C for 4 h at ZT20-24 before harvesting for total RNA extraction. Gene expression levels were normalized to APX3 and presented as values relative to that of wild type (WT) kept at 20 °C. Error bars indicate s.d. (n = 3). The P-values for the interaction (genotype x temperature) are shown at the top. Source data are provided as a Source Data file.