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. 2020 Feb 26;10:3543. doi: 10.1038/s41598-020-60635-w

Figure 2.

Figure 2

Protein interactions of MBW factors by bimolecular fluorescence complementation assays (BiFC). N. benthamiana leaves agroinfiltrated with different combinations of MBW factors fused to NtYFP or CtYFP peptides in Ct (right yellow label) or Nt (left yellow label) positions together with nucleus/nucleolus markers (a). The green reconstituted fluorescence (YFP), red (nucleus/nucleolus marker), transmitted light (TL) channels and merged images are shown in the figure. A diagram of previous yeast two-hybrid (orange lines) and positive BiFC interactions obtained in this study (green lines) (b). The fluorescent signals were visualized at 72 hours post-infiltration. Scale bars are shown in merged images.