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. 2020 Feb 26;10:3464. doi: 10.1038/s41598-020-60340-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression patterns of 7 MCO genes in ovaries and eggs of P. stali in terms of cDNA copies per ribosomal protein L32 (rpL32) cDNA copy. (A) PsMCO1 (Pslaccase1). (B) PsMCO2 (Pslaccase2). (C) PsMCO3. (D) PsMCO4. (E) PsMCO5. (F) PsMCO6 (PsMCORP). (G) PsMCO7. Immature ovaries (Im) were collected from newly emerged adults, whereas mature ovaries (M) and fully developed oocytes (Dev.) were collected from 14-day-old adults. Egg samples were collected daily after oviposition (1d–5d). Numbers above bars are sample size (i.e. biological replicates).