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. 2020 Feb 26;10:3447. doi: 10.1038/s41598-020-60273-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological analysis of wild type (WT) and LRRK2 G2019S TH+ dopaminergic neurons. (a) Representative TH+ neuron and (b) traced skeleton for morphological quantification. (c) Average number of neurites per cell, (d) average number of branches per cell, (e) average neurite length per cell, (f) average branch length per cell, (g) length per neurite, and (h) branches per neurite were quantified in WT and LRRK2 G2019S Cj-ESC and Cj-iPSC TH+/MAP2+ d67 dopaminergic neurons; n = 25–45 neurons per line. (One-way ANOVA with Tukey’s multiple comparison was used to compare between Cj-ESC lines or between Cj-iPSC lines; p < 0.05^*, 0.01^**).