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. 2020 Feb 26;10:3447. doi: 10.1038/s41598-020-60273-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ROS and viability assays of wild type and LRRK2 G2019S neurons. (a) Cj-ESCs, and (b) Cj-iPSCs assayed for ROS levels after treatment with vehicle, 0.1%, 0.5%, and 1.0% hydrogen peroxide after 20 days of maturation (Graph key represents each column of the figure). (c) Cj-ESCs and (d) Cj-iPSCs assayed for ROS levels after an additional 20 days of culture. All ROS graphs are shown as a concentration of DCF compound per cell as a readout of intracellular ROS abundance. (e) Cj-ESCs and (f) Cj-iPSCs assayed for viability after treatment with 0.1%, 0.5% hydrogen peroxide, or 5% dimethylsulfoxide after 40 days of maturation. Viability was normalized to control wells treated with vehicle; n = 3 separate wells per line and condition. (Two-way ANOVA with Bonferroni correction comparing cell lines to condition with significance presented on the graphs only in comparison to wild type: p < 0.05^*, 0.01^Φ, 0^.001^Ω, 0.0001^Ψ).