Fig. 1.

Immunological responses. a KLH-specific T cell proliferation was measured before the start of therapy and after each vaccination of the first cycle in PBMCs of melanoma patients. The proliferative response to KLH is depicted as the highest proliferation index (proliferation with KLH/proliferation without KLH) observed during the first cycle. b PBMCs were tested for TM⁺ CD8⁺ T cells recognizing gp100 or tyrosinase in HLA-A2.1 positive patients. c SKILs were tested for TM⁺ CD8⁺ T cells recognizing gp100 or tyrosinase in HLA-A2.1 positive patients and d a functional T cell response in all patients. *p < 0.001, DC dendritic cell, KLH Keyhole limpet hemocyanin, ns not significant, PBMC peripheral blood mononuclear cell, SKIL skin-test infiltrating lymphocytes, TM tetramer