Figure 3.

Verification of transgene integration and expression of PmCesA2 into the poplar genome. (A) Integration of PmCesA2 poplar transgenic lines using PCR amplification. Genomic DNA was extracted from the leaves of 1-month-old transgenic poplars. The PCR products were assessed through electrophoresis on 1.0% agarose gel. (B) Relative expression of PmCesA2 in transgenic poplars by real-time PCR. Expressed levels were averaged ( ± SE) from three different samples per line. Actin served as the internal reference. * denotes significance at p < 0.05.