Fig. 3. Taxonomic and functional composition of vaginal microbiome in VIRGO.

a Top 20 species with the most abundant gene content in VIRGO. The logarithm of the ratio of the gene content of a species over the entire community to the base 2. Plotted are interquartile ranges (IQRs, boxes), medians (line in box), and mean (red diamond). b Species-specific metagenome accumulation curves for the number of non-redundant genes. c Functional distribution of non-redundant genes in VIRGO. Functional categories were defined using EggNOG (v4.5)⁵⁹. d Prevalence of BVAB1 in metagenomes using a minimum number of genes threshold of 50% of the estimated BVAB1 genome size. A gene was present if ≥3 reads mapped to it. e Relationship between the depth of sequencing and the number of bacterial non-redundant genes identified using VIRGO. Each point is a separate metagenome and is color-coded according to community state type.