Fig. 5. Demonstration using VIRGO and VOG to study vaginal microbiome.
a 4 sampling points were selected based on a longitudinally profiled subject prior to (T1), during (T2 and T3), and after (T4) an episode of bacterial vaginosis using 16S rRNA profiling. b Functional profiling of the metagenome (MG) and metatranscriptome (MT) of each of the four sampling points. Functional categories were annotated using EggNOG (v4.5)59. c Functional profiles stratified by species using the taxonomic profiling provided by VIRGO. d Demonstrative use of VOG to characterize the G. vaginalis cholesterol-dependent cytolysin (CDC) protein family. It shows the phylogeny of CDC-containing protein and alignment of domain 4 of the CDCs that is generally well conserved but contains a single divergent site, highlighted in yellow.