Fig. 2. POGZ regulates the mouse cortical neuronal development.

a Temporal expression patterns of Pogz mRNA in the brain (each n = 3). E embryonic day; wk week-old. b Regional expression pattern of Pogz mRNA in the E16.5 brain (each n = 3). VZ ventricular zone; SVZ subventricular zone. c Fluorescence in situ hybridization of coronal sections of the E16.5 brain for Pogz and immunostaining with antibodies against PAX6, SOX2, and TBR2. Scale bars, 100 μm. LV lateral ventricle; VZ ventricular zone; SVZ subventricular zone; IZ intermediate zone. d Impairment of neuronal migration by shRNA-mediated knockdown of Pogz in E18.5 mouse cortices electroporated at E14.5. CP cortical plate; IZ intermediate zone; SVZ subventricular zone. Scale bars, 50 μm. e Quantification of GFP⁺ cells in each layer (each n = 4). f GFP⁺ neurons were co-labeled for SATB2 (a layer II/III marker). g Quantification of GFP⁺ SATB2⁺ neurons (n = 4). h GFP⁺ neurons were co-labeled for CTIP2 (a layer V marker). i Quantification of GFP⁺ CTIP2⁺ neurons (n = 4). j GFP⁺ neurons were co-labeled for TBR1 (a layer VI marker). k Quantification of GFP⁺ TBR1⁺ neurons (n = 4). Note that Pogz knockdown had little effect on the proportion of SATB2⁺ GFP⁺, CTIP2⁺ GFP⁺ or TBR1⁺ GFP⁺ neurons and that the GFP⁺ cells with delayed migration were mostly SATB2⁺ neurons. h Same slice as in f. f, h, j Right panels, magnifications of the areas outlined with orange boxes. White scale bars, 50 μm; orange scale bars, 10 μm. a, b, g, i, k One-way ANOVA with Bonferroni–Dunn post hoc tests; a F_{10, 22} = 48.52; b F_{7, 16} = 73.23; g F_{2, 9} = 0.393; i F_{2, 9} = 1.079; k F_{2, 9} = 0.239. e Two-way repeated-measures ANOVA with Bonferroni–Dunn post hoc tests, F_{4, 27} = 39.28. ***P < 0.001. Data are presented as the mean ± s.e.m.