Fig. 3. POGZ regulates the neuronal differentiation of mouse cortical neural stem cells.

a Slight, non-significant migration defects caused by shRNA-mediated knockdown of Pogz in E16.5 mouse cortices electroporated at E14.5. CP cortical plate; IZ intermediate zone; SVZ subventricular zone; VZ ventricular zone; E embryonic day. Scale bars, 50 μm. b Quantification of GFP⁺ cells in each layer (each n = 4). CP cortical plate; IZ intermediate zone; SVZ subventricular zone; VZ ventricular zone. c Increased number of PAX6⁺ NSCs caused by Pogz knockdown in E16.5 mouse cortices electroporated at E14.5. d Quantification of PAX6⁺ cells (each n = 4). e Decreased number of TBR2⁺ differentiated IPs caused by Pogz knockdown in E16.5 mouse cortices electroporated at E14.5. f Quantification of TBR2⁺ cells (each n = 4). g Decreased number of SATB2⁺ differentiated neurons caused by Pogz knockdown in E16.5 mouse cortices electroporated at E14.5. h Quantification of SATB2⁺ cells (each n = 4). c, e, g Right panels, magnifications of the areas outlined with orange boxes. Arrowheads indicate co-labeled cells. White scale bars, 50 μm; orange scale bars, 10 μm. b Two-way repeated-measures ANOVA with Bonferroni–Dunn post hoc tests, F_{4, 27} = 1.861. d, f, h One-way ANOVA with Bonferroni–Dunn post hoc tests; d F_{2, 9} = 18.37; f F_{2, 9} = 5.710; h F_{2, 9} = 11.91. *P < 0.05, **P < 0.01. Data are presented as the mean ± s.e.m.