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. 2020 Feb 26;11:1049. doi: 10.1038/s41467-020-14877-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Commercial ESI-MS (LTQ Orbitrap Elite, Thermo Scientific), b bulk phase at 37 °C for 14 h, followed by analysis with commercial ESI-MS, and ESSI-MS applied with c a positive high voltage (+3 kV), and d a negative high voltage (−3 kV). Magenta asterisks denote the peptide fragments, and blue #s mark undigested myoglobin peaks.