Table 1.

Comparisons of various techniques for accelerated protein digestion⁶⁸.

Accelerated technique	Online	Digestion time
High temperature	possible	∼15 min
Microwave	possible	≤15 min
Ultrasound	Not feasible	≤5 min
High pressure	Yes	<1 min
Infrared	Not done	∼5 min
Organic solvent	Not done	≤5 h
On-column immobilized enzyme	Yes	<6 min
On-chip immobilized enzyme	Yes	5 s
Magnetic particle immobilized enzyme	Yes	∼30 s
Microdroplets (this work)	Yes	<1 ms

Note: Digestion time refers to digestion reaction time, not the total experiment time.