Fig. 3.

Crb2b is expressed in polarised cells of the ICA at 5 dpf. Immunostaining of transverse cryosections through the eye. (A,B,E,F) Overview of a WT (A,E) and a mutant (B,F) eye at 5 dpf. Higher magnifications of the boxed regions in the dorsal ICA are shown in C–D‴′ (WT) and G–H‴′ (crb2b^e40). Higher magnifications of the boxed regions in the ventral ICA are shown in Fig. S4. Crb2b staining (anti-Crb2b^e8e9) is detected in a cluster of cells in the WT ICA (C) but not in the mutant ICA (D). F-actin (C′,D′; visualised by phalloidin), aPKC (G,H) and ZO-1 (G′,H′) appear apically enriched (C′,D′). Arrows point to the cluster of polarised Crb2b expressing cells, and asterisks mark the lens. dICA, dorsal iridocorneal angle; Ep, epidermis; CMZ, ciliary marginal zone. Scale bars: (A,B,E,F) 50 µm; (C–D‴′, G–H‴′) 10 µm.