Fig. 6.
SAX-7 can act both in neurons and glia to promote dendrite extension. (A,B) Transgenes containing SAX-7S cDNA under the control of promoters expressed in neurons, glia, both together, or no transgene (−, same data as Fig. 3), were introduced into sax-7 animals. (A) URX and (B) BAG dendrite lengths were measured in each strain as a percentage of the distance from cell body to nose. Colored bars represent individual dendrites (n≥49 per genotype); black bars represent population averages. P-values (Wilcoxon Rank Sum test) compared with sax-7 with no transgene (−) are shown at the top. Rescue was greater when SAX-7S cDNA was expressed in neurons and glia together compared with neurons only (URX, P<10−5; BAG, P<10−3) or glia only (URX, P<10−4; BAG, P<10−5). Shaded region represents wild-type mean±5 s.d. for each neuron type and the percentage of dendrites in this range (‘full-length dendrites’) is indicated below the plots. (C) Single-wavelength and merged images of animals expressing SAX-7S-YFP and the membrane marker myristyl-mCherry under control of a glia-specific promoter (itx-1pro). Inset shows that SAX-7S-YFP is sometimes enriched in glial endings, where the glia contact URX and BAG.